highyield t7 cy3 rna labelling kit (Jena Bioscience)

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Jena Bioscience highyield t7 cy3 rna labelling kit

(A) Representative immunofluorescence images showing the localization nascent transcripts (EU, green) inside the nucleus (blue) of early GV oocytes. Images show EU levels in negative control (no EU), control (with EU), upon treatment with α-amanitin, and triptolide. Scale bar: 10 µm. (B) Frequency of PSSC with α-amanitin measured by scoring the percentage of eggs with PSSC, similar to levels observed with triptolide . Plots show number of eggs analyzed on top. Statistical significance is measured by Fisher’s exact test, ns = not significant. (C) Representative time-lapse images showing the localization of MajSat RNA in GV and MI oocytes and MII eggs upon microinjection of GV oocytes with <t>UTP-X-Cy3</t> labeled MajSat (top panel) and control (bottom panel) RNA. MajSat RNA localizes to the pericentromeres during metaphase I and metaphase II stages along with foci present in the cytoplasm. Cy3-labeled control RNA localizes only in the cytoplasm and not on chromosomes. MajSat and control RNA (green), chromosomes (H2B-SNAP, blue) are shown. Scale bars: 10 µm. (D) Co-localization of MajSat RNA and SGO1 observed on metaphase I chromosome spreads upon performing RNA FISH with immunofluorescence. Scale bar: 10 µm.

Highyield T7 Cy3 Rna Labelling Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/highyield+t7+cy3+rna+labelling+kit/HighYield+T7+Cy3+RNA+Labeling+Kit/bio_rxiv__64898__2026__01__08__698387-185-1-7
Average 94 stars, based on 5 article reviews

highyield t7 cy3 rna labelling kit - by Bioz Stars, 2026-10

94/100 stars

Images

1) Product Images from "Restoring Shugoshin 1 reduces chromosome errors in human eggs"

Article Title: Restoring Shugoshin 1 reduces chromosome errors in human eggs

Journal: bioRxiv

doi: 10.64898/2026.01.08.698387

Figure Legend Snippet: (A) Representative immunofluorescence images showing the localization nascent transcripts (EU, green) inside the nucleus (blue) of early GV oocytes. Images show EU levels in negative control (no EU), control (with EU), upon treatment with α-amanitin, and triptolide. Scale bar: 10 µm. (B) Frequency of PSSC with α-amanitin measured by scoring the percentage of eggs with PSSC, similar to levels observed with triptolide . Plots show number of eggs analyzed on top. Statistical significance is measured by Fisher’s exact test, ns = not significant. (C) Representative time-lapse images showing the localization of MajSat RNA in GV and MI oocytes and MII eggs upon microinjection of GV oocytes with UTP-X-Cy3 labeled MajSat (top panel) and control (bottom panel) RNA. MajSat RNA localizes to the pericentromeres during metaphase I and metaphase II stages along with foci present in the cytoplasm. Cy3-labeled control RNA localizes only in the cytoplasm and not on chromosomes. MajSat and control RNA (green), chromosomes (H2B-SNAP, blue) are shown. Scale bars: 10 µm. (D) Co-localization of MajSat RNA and SGO1 observed on metaphase I chromosome spreads upon performing RNA FISH with immunofluorescence. Scale bar: 10 µm.

Techniques Used: Immunofluorescence, Negative Control, Control, Microinjection, Labeling

In Vitro:

Article Title: The Hox transcription factor Ultrabithorax binds RNA and regulates co-transcriptional splicing through an interplay with RNA polymerase II
Article Snippet: .. For producing internally labelled RNA, in vitro transcription was performed using the HighYield T7 Cy3 RNA Labelling Kit (Jena Bioscience, RNT-101-CY3) in accordance to the instructions of the manufacturer. .. Each reaction contained 500 ng DNA template, 0.4 μl Cy3-UTP (5 mM) and 0.4 μl RiboLock RNase Inhibitor (Thermo Fisher Scientific) and was incubated for 1 h at 37°C.

Article Title: The Hox transcription factor Ultrabithorax binds RNA and regulates co-transcriptional splicing through an interplay with RNA polymerase II
Article Snippet: .. For producing internally labelled RNA, in vitro transcription was performed using the HighYield T7 Cy3 RNA Labelling Kit (Jena Bioscience, RNT-101-CY3) in accordance to the instructions of the manufacturer. .. Each reaction contained 500 ng DNA template, 0.4 μl UTP-X-Cy3 (5 mM) and 0.4 μl RiboLock RNase Inhibitor (ThermoFisher) and was incubated for 1 hour at 37°C.

Amplification:

Article Title: Restoring Shugoshin 1 reduces chromosome errors in human eggs
Article Snippet: .. The HighYield T7 Cy3 RNA Labelling Kit (Jena Bioscience, #RNT-101-CY3) was used to label amplified sequences with Cy3-labelled UTP (UTP-X-Cy3, 35%), followed by DNA removal using TurboTM DNAse (ThermoFisher). .. Purification of Cy3-uridine-labelled RNA was performed using Monarch® RNA Cleanup Kit (NEB, #T2040L).

other:

Article Title: Synergistic DNA and RNA binding of the Hox transcription factor Ultrabithorax coordinates splicing and shapes in vivo homeotic functions
Article Snippet: For in vitro transcription, we employed selected fragments of alternatively spliced exons that were cloned into the pGEM-T Easy (Promega) Vector (listed in ).

Article Title: Synergistic DNA and RNA binding of the Hox transcription factor Ultrabithorax coordinates splicing and shapes in vivo homeotic functions.
Article Snippet: In vitro interaction assays were performed with equal amounts of GST or GST fusion proteins in affinity buffer (20 mM HEPES, 10 μM ZnCl 2 , 0.1% Triton, 2 mM EDTA) supplemented with NaCl, 1 mM DTT, 0.1 mM PMSF and protease inhibitor cocktail (Sigma).

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